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    Quick Facts

    Medium Of InstructionsMode Of LearningMode Of Delivery
    EnglishSelf StudyVideo and Text Based

    Courses and Certificate Fees

    Fees InformationsCertificate AvailabilityCertificate Providing Authority
    INR 1000yesIIT Bombayyr

    The Syllabus

    Basics of Proteins and Proteomics
    • Introduction to amino acids
    • Introduction to Proteins
    • Protein folding & misfolding
    • Introduction to Proteomics
    • Lab session – Protein-protein interaction using label-free biosensors

    Gel-based proteomics
    • Sample preparation and pre-analytical factors
    • Sample preparation: Pre-analytical factors (contd.)
    • Sample preparation: Protein extraction and quantification
    • One-dimensional electrophoresis
    • Introduction to 2-DE

    Two-dimensional gel electrophoresis (2-DE)
    • 2-DE: Second dimension, staining & destaining
    • 2-DE: Gel analysis
    • 2-DE Applications
    • 2-DE Applications (contd.) & Challenges
    • Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR & data analysis

    Difference in gel electrophoresis (DIGE) & Systems Biology
    • 2D-DIGE: Basics
    • 2D-DIGE: Data analysis
    • 2D-DIGE: Applications
    • Systems biology and proteomics – I
    • Systems biology and proteomics - II

    Basics of mass spectrometry
    • Fundamentals of mass spectrometry
    • Chromatography technologies
    • Liquid chromatography
    • Mass spectrometry: Ionization sources
    • Mass spectrometry: Mass analyzers

    Basics of mass spectrometry and sample preparation
    • MALDI sample preparation and analysis
    • Hybrid mass spectrometry configurations
    • Lab session - Demonstration of Q-TOF MS technology
    • In-gel & in-solution digestion
    • Lab session - Sample preparation: tissue sample preservation technology

    Quantitative proteomics
    • Introduction to quantitative proteomics
    • SILAC: In vivo labelling
    • iTRAQ: In vitro labelling
    • TMT: In vitro labelling
    • Quantitative proteomics data analysis

    Advancement in Proteomics
    • Proteomics applications
    • Challenges in proteomics
    • OMICS and translational research
    • Lab session – Targeted proteomics using triple quadrupole mass spectrometry
    • Lab session – Targeted proteomics: multiple reaction monitoring

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