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Introduction to Proteomics
Online
8 WeeksQuick Facts
| Medium Of Instructions | Mode Of Learning | Mode Of Delivery |
|---|---|---|
| English | Self Study | Video and Text Based |
Courses and Certificate Fees
| Fees Informations | Certificate Availability | Certificate Providing Authority |
|---|---|---|
| INR 1000 | yes | IIT Bombayyr |
The Syllabus
Basics of Proteins and Proteomics
- Introduction to amino acids
- Introduction to Proteins
- Protein folding & misfolding
- Introduction to Proteomics
- Lab session – Protein-protein interaction using label-free biosensors
Gel-based proteomics
- Sample preparation and pre-analytical factors
- Sample preparation: Pre-analytical factors (contd.)
- Sample preparation: Protein extraction and quantification
- One-dimensional electrophoresis
- Introduction to 2-DE
Two-dimensional gel electrophoresis (2-DE)
- 2-DE: Second dimension, staining & destaining
- 2-DE: Gel analysis
- 2-DE Applications
- 2-DE Applications (contd.) & Challenges
- Lab session - Protein/peptide pre-fractionation using OFFGEL FRACTIONATOR & data analysis
Difference in gel electrophoresis (DIGE) & Systems Biology
- 2D-DIGE: Basics
- 2D-DIGE: Data analysis
- 2D-DIGE: Applications
- Systems biology and proteomics – I
- Systems biology and proteomics - II
Basics of mass spectrometry
- Fundamentals of mass spectrometry
- Chromatography technologies
- Liquid chromatography
- Mass spectrometry: Ionization sources
- Mass spectrometry: Mass analyzers
Basics of mass spectrometry and sample preparation
- MALDI sample preparation and analysis
- Hybrid mass spectrometry configurations
- Lab session - Demonstration of Q-TOF MS technology
- In-gel & in-solution digestion
- Lab session - Sample preparation: tissue sample preservation technology
Quantitative proteomics
- Introduction to quantitative proteomics
- SILAC: In vivo labelling
- iTRAQ: In vitro labelling
- TMT: In vitro labelling
- Quantitative proteomics data analysis
Advancement in Proteomics
- Proteomics applications
- Challenges in proteomics
- OMICS and translational research
- Lab session – Targeted proteomics using triple quadrupole mass spectrometry
- Lab session – Targeted proteomics: multiple reaction monitoring